WB
H
Endogenous
50
Rabbit IgG
#P11926
4953
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein of human ornithine decarboxylase/ODC protein.
Background
Ornithine decarboxylase (ODC) is a rate-limiting enzyme in polyamine biosynthesis. Studies found that ODC and polyamine levels are often increased, contributing to cancer cell proliferation (1). Deficiency of the ODC enzyme suppresses acute myeloid leukemia cell proliferation (2). In addition, oncogene products YAP and TAZ activate transcription of the ODC1 gene that encodes the ODC enzyme, leading to more polyamine biosynthesis. Enhanced polyamine levels mediate tumor growth driven by YAP and TAZ via a polyamine-eIF5A hypusination-LSD1 axis (3). Furthermore, continual efferocytosis of apoptotic cells by macrophages is promoted by converting arginine and ornithine, both from apoptotic cells, to putrescine by arginase-1 and ODC in macrophages. Putrescine enhances subsequent rounds of efferocytosis (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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