Organelle Localization IF Antibody Sampler Kit (#8653) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
AIF (D39D2) XP^® Rabbit mAb	5318	20 µl	67 kDa	Rabbit IgG
EEA1 (C45B10) Rabbit mAb	3288	20 µl	170 kDa	Rabbit IgG
LAMP1 (D2D11) XP^® Rabbit mAb	9091	20 µl	42 (non-glycosylated), 90-120 (glycosylated) kDa	Rabbit IgG
PDI (C81H6) Rabbit mAb	3501	20 µl	57 kDa	Rabbit
RCAS1 (D2B6N) XP^® Rabbit mAb	12290	20 µl	32 kDa	Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or two western blots with each antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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