WB
H
Endogenous
37
Rabbit IgG
#Q96E52
115209
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu504 of human OMA1 protein.
Background
Mitochondria continuously divide and fuse. This dynamic process is highly regulated in response to various physiological cues (1,2). The GTPase OPA1 mediates the fusion of the mitochondrial inner membrane. Constitutive proteolytic processes mediated by OMA1 (S1 site) and YME1L (S2 site) convert long isoforms (L-OPA1) into short isforms (S-OPA1). The balance between L-OPA1 and S-OPA1 is required to maintain a normal morphology of mitochondria (3,4).OMA1 is synthesized as a precursor and processed into a mature form (5,6). OMA1 is constitutively active and cleaves L-OPA1 at the S1 site. However, various stress stimuli can further activate OMA1 and result in the rapid and complete conversion of L-OPA1 into S-OPA1, which inhibits fusion and causes mitochondrial fragmentation (6).
- Chan, D.C. (2012) Annu Rev Genet 46, 265-87.
- Lee, H. and Yoon, Y. (2016) Biochem Soc Trans 44, 1725-1735.
- Anand, R. et al. (2014) J Cell Biol 204, 919-29.
- MacVicar, T. and Langer, T. (2016) J Cell Sci 129, 2297-306.
- Head, B. et al. (2009) J Cell Biol 187, 959-66.
- Baker, M.J. et al. (2014) EMBO J 33, 578-93.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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