WB, IHC-P
H
Endogenous
35-42
Rabbit IgG
#P78380
4973
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #20752.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val230 of human OLR1/LOX1 protein.
Background
Oxidatively modified low-density lipoprotein (Ox-LDL) causes endothelial cell dysfunction and activation and contributes to atherosclerosis. Oxidized low-density lipoprotein receptor 1 (OLR1), also known as lectin-like oxidized LDL receptor 1 (LOX1), was cloned and identified as a cell membrane receptor for Ox-LDL in vascular endothelial cells (1). In addition, research studies show that OLR1/LOX1 is one of the most overexpressed genes in human polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) that regulate immune responses in cancer. OLR1/LOX1 is a specific marker of human PMN-MDSC (2). Furthermore, OLR1/LOX1 was shown to be upregulated in esophageal cancer. OLR1/LOX1 deficiency stimulates autophagic cell death, inhibiting esophageal cancer development (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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