| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| CHD3 Antibody | 4241 | 20 µl | 260 kDa | Rabbit |
| CHD4 (D8B12) Rabbit mAb | 11912 | 20 µl | 260 kDa | Rabbit IgG |
| HDAC1 (10E2) Mouse mAb | 5356 | 20 µl | 62 kDa | Mouse IgG1 |
| HDAC2 (3F3) Mouse mAb | 5113 | 20 µl | 60 kDa | Mouse IgG1 |
| MBD3 (N87) Antibody | 14540 | 20 µl | 32, 34 kDa | Rabbit |
| MTA1 (D40D1) XP® Rabbit mAb | 5647 | 20 µl | 78-82 kDa | Rabbit IgG |
| RBAP46 (V415) Antibody | 6882 | 20 µl | 48 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
NuRD Complex Antibody Sampler Kit offers an economical means of detecting each target protein that composes the nucleosome remodeling and deacetylation complex (NuRD). The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.
Storage
Background
The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11).
- Tong, J.K. et al. (1998) Nature 395, 917-21.
- Xue, Y. et al. (1998) Mol Cell 2, 851-61.
- Zhang, Y. et al. (1998) Cell 95, 279-89.
- Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
- Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
- Fujita, N. et al. (2003) Cell 113, 207-19.
- Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
- Ng, H.H. et al. (1999) Nat Genet 23, 58-61.
- Zhang, Y. et al. (1997) Cell 89, 357-64.
- Verreault, A. et al. (1998) Curr Biol 8, 96-108.
- Wade, P.A. et al. (1999) Nat Genet 23, 62-6.
Background References
Trademarks and Patents
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