WB, IP
H M
Endogenous
43
Rabbit IgG
#P10588
2063
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NR2F6/EAR-2 protein.
Background
NR2F6 (Nuclear receptor subfamily 2 group F member 6, EAR-2, COUP-TFIII) is a member of the NR2F family of transcription factors, defined by their role in the regulation of organogenesis, neurogenesis, and cellular differentiation during embryonic development (1,3). NR2F6 acts as a negative regulator for TRβ1-mediated transcriptional activity, a transcriptional repressor of IL-17 expression in Th-17 differentiated CD4+ T cells, and a repressor of the NFAT/AP-1 complex on both the interleukin-2 and the interferon-γ cytokine promoters (2-5). NR2F6 has a non-redundant role in antagonizing anti-cancer effector T cell responses (5). It has been shown that loss of NR2F6 enhances the activity of established PD-L1 checkpoint blockade, promoting tumor regression and increased survival in mouse models (5,6).
- Ladias, J.A. et al. (1992) J Biol Chem 267, 15849-60.
- Zhu, X.G. et al. (2000) Mol Cell Biol 20, 2604-18.
- Hermann-Kleiter, N. et al. (2008) Immunity 29, 205-16.
- Hermann-Kleiter, N. and Baier, G. (2014) Cell Commun Signal 12, 38.
- Hermann-Kleiter, N. et al. (2015) Cell Rep 12, 2072-85.
- Klepsch, V. et al. (2018) Nat Commun 9, 1538.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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