Revision 1

#65507Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

7-17

SOURCE:

Rabbit

UniProt ID:

#Q16617

Entrez-Gene Id:

4818

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

NKG7 Antibody recognizes endogenous levels of total NKG7 protein. This antibody may recognize multiple predicted isoforms of NKG7 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly161 of human NKG7 protein. Antibodies are purified by peptide affinity chromatography.

Background

Natural killer cell granule protein 7 (NKG7, also known as GMP-17 and GIG-I) is a novel type 1 integral membrane protein expressed at high levels in lymphocytes, including natural killer (NK) cells, γδ T cells, a subset of αβ T cells, lymphokine-activated killer (LAK) cells (1), and activated T regulatory cells (2). NKG7 expression can be induced in neutrophils following GM-CSF treatment (3). NKG7 protein is localized to the membranes of cytotoxic granules. Following target cell-induced NK cell degranulation, NKG7 translocates from granules to the plasma membrane (4). Studies have also shown that NKG7 is significantly upregulated in allograft rejection patients (5-8).

  1. Turman, M.A. et al. (1993) Hum Immunol 36, 34-40.
  2. Sugimoto, N. et al. (2006) Int Immunol 18, 1197-209.
  3. Shimane, M. et al. (1994) Biochem Biophys Res Commun 199, 26-32.
  4. Medley, Q.G. et al. (1996) Proc Natl Acad Sci U S A 93, 685-9.
  5. Sacreas, A. et al. (2018) PLoS One 13, e0205107.
  6. Sigdel, T.K. et al. (2015) PLoS One 10, e0138133.
  7. Ashokkumar, C. et al. (2011) Am J Pathol 179, 1929-38.
  8. Meehan, S.M. et al. (1997) Lab Invest 76, 639-49.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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