WB
H
Endogenous
62, 75
Rabbit
#Q96NY8
81607
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Nectin-4/PVRL4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Nectin-4/poliovirus receptor-related 4 (PVRL4) is a type-I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and promotes cell-cell adhesion by serving as a major component of adherens junctions (1-3). The extracellular domain of Nectin-4, which contains an Ig variable-like domain (V) and two Ig constant-like domains (C), mediates binding to the measles virus (4) and to neighboring cells through trans heterophilic interactions with Nectin-1 (5,6). Unlike other nectin family members, which are widely expressed in adult tissues, Nectin-4 expression in humans is largely restricted to the placenta (7). Research studies have demonstrated that Nectin-4 is overexpressed in a variety of human solid tumors of the pancreas (8), breast (9,10), lung (11), and ovary (12). Due to its restricted expression pattern in normal human tissues, Nectin-4 may serve as a novel diagnostic and therapeutic target for a variety of human tumors.
- Takai, Y. et al. (2008) Annu Rev Cell Dev Biol 24, 309-42.
- Takai, Y. and Nakanishi, H. (2003) J Cell Sci 116, 17-27.
- Harrison, O.J. et al. (2012) Nat Struct Mol Biol 19, 906-15.
- Mühlebach, M.D. et al. (2011) Nature 480, 530-3.
- Mateo, M. et al. (2014) J Virol 88, 14161-71.
- Fabre, S. et al. (2002) J Biol Chem 277, 27006-13.
- Reymond, N. et al. (2001) J Biol Chem 276, 43205-15.
- Nishiwada, S. et al. (2015) J Exp Clin Cancer Res 34, 30.
- Lattanzio, R. et al. (2014) Oncogenesis 3, e118.
- Fabre-Lafay, S. et al. (2007) BMC Cancer 7, 73.
- Takano, A. et al. (2009) Cancer Res 69, 6694-703.
- Nabih, E.S. et al. (2014) Biomarkers 19, 498-504.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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