WB, IP
H M R Mk
Endogenous
45
Rabbit
#Q9UGV2
57446
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NDRG3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors (4,5).
Members of the NDRG family each have distinct expression patterns; NDRG3 is most highly expressed in the testis, prostate, ovary, and brain (2,6,7). NDRG3 has also been shown to be up-regulated by androgen in prostate cell lines and can promote cell growth in those cells (8).
- Shimono, A. et al. (1999) Mech Dev 83, 39-52.
- Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
- Zhou, R.H. et al. (2001) Genomics 73, 86-97.
- Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
- Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
- Okuda, T. and Kondoh, H. (1999) Biochem Biophys Res Commun 266, 208-15.
- Zhao, W. et al. (2001) Biochim Biophys Acta 1519, 134-8.
- Wang, W. et al. (2009) Int J Cancer 124, 521-30.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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