WB
H M R
Endogenous
45
Rabbit
#Q9UN36
57447
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys25 of human NDRG2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors (4,5).
Expression of NDRG1 and NDRG2 is up-regulated by p53 and HIF-1, and contributes to apoptosis driven by those pathways (6-11). NDRG1 and NDRG2 are also associated with pathological conditions in the nervous system. Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (12, 13). Elevated expression of NDRG2 has been observed with Alzheimer's disease (14). Both NDGR1 and NDGR2 are phosphorylated at multiple sites by Akt and/or SGK1, although the precise physiological role of this phosphorylation is not known (15, 16).
- Shimono, A. et al. (1999) Mech Dev 83, 39-52.
- Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
- Zhou, R.H. et al. (2001) Genomics 73, 86-97.
- Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
- Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
- Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
- Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
- Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
- Cangul, H. (2004) BMC Genet 5, 27.
- Wang, L. et al. (2008) Cell Physiol Biochem 21, 239-50.
- Liu, N. et al. (2008) Nucleic Acids Res 36, 5335-49.
- Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
- Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
- Mitchelmore, C. et al. (2004) Neurobiol Dis 16, 48-58.
- Murray, J.T. et al. (2004) Biochem J 384, 477-88.
- Burchfield, J.G. et al. (2004) J Biol Chem 279, 18623-32.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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