WB, IP, IF-IC, FC-FP
H M
Endogenous
120
Rabbit IgG
#Q14596
4077
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys601 of human NBR1 protein.
Background
Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in selective autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5-11).
- Campbell, I.G. et al. (1994) Hum Mol Genet 3, 589-94.
- Miki, Y. et al. (1994) Science 266, 66-71.
- Lange, S. et al. (2005) Science 308, 1599-603.
- Müller, S. et al. (2006) FEBS Lett 580, 341-4.
- Kirkin, V. et al. (2009) Mol Cell 33, 505-16.
- Lamark, T. et al. (2009) Cell Cycle 8, 1986-90.
- Kenific, C.M. and Debnath, J. (2016) Autophagy 12, 1958-1959.
- Hafrén, A. and Hofius, D. (2017) Autophagy 13, 2000-2001.
- Werner, A. et al. (2019) Autophagy 15, 78-97.
- Sánchez-Martín, P. et al. (2020) EMBO Rep 21, e48902.
- Turco, E. et al. (2021) Nat Commun 12, 5212.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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