Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IF-IC

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#O96009

Entrez-Gene Id:

9476

Product Information

Product Usage Information

Application Dilution
Immunofluorescence (Immunocytochemistry) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Napsin A (D2G1Y) Rabbit mAb (IF Formulated) recognizes endogenous levels of total napsin A protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human napsin A protein.

Background

Napsin A is an aspartic proteinase that is expressed in normal lung and kidney (1). In the lung, napsin A is expressed by type II pneumocytes and alveolar macrophages, where it plays a role in processing surfactant protein B (2). Napsin A is expressed in lung adenocarcinomas, where it can be used to identify primary and metastatic lesions with greater sensitivity compared to TTF-1 (3,4). Napsin A expression has also been described in other types of cancer, such as kidney and thyroid cancer (5).

  1. Tatnell, P.J. et al. (1998) FEBS Lett 441, 43-8.
  2. Schauer-Vukasinovic, V. et al. (1999) FEBS Lett 462, 135-9.
  3. Jagirdar, J. (2008) Arch Pathol Lab Med 132, 384-96.
  4. Turner, B.M. et al. (2012) Arch Pathol Lab Med 136, 163-71.
  5. Bishop, J.A. et al. (2010) Hum Pathol 41, 20-5.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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