WB
H M R Mk
Endogenous
60
Rabbit IgG
#Q13564
8883
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala179 of human NAE1/APPBP1 protein.
Background
Similar to ubiquitin, NEDD8 is covalently linked to target proteins through an enzymatic cascade composed of NEDD8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for NEDD8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 are cullin-based RING E3 ligases. Neddylation of cullin isoforms activates the related ubiquitin E3 complex by promoting its interaction with a cognate ubiquitin-E2 ligase (6-7). Neddylation of Cul-1 complexes containing βTrCP and SKP2 has been shown to be required for controlling the stability of important signaling targets such as IκB, NF-κB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11).
NAE1/APPBP1 (NEDD8-Activating Enzyme 1/Amyloid Beta Precursor Protein Binding Protein 1) exists in a heterodimeric complex with UBA3. This complex functions as an E1 NEDD8-activating enzyme, which utilizes ATP to adenylate the C-terminal glycine of NEDD8 (12-14). Research studies suggest that inhibition of the APPBP1-UBA3 complex may be of therapeutic value for the treatment of human cancers (15,16).
- Huang, D.T. et al. (2007) Nature 445, 394-8.
- Huang, D.T. et al. (2005) Mol Cell 17, 341-50.
- Liakopoulos, D. et al. (1998) EMBO J 17, 2208-14.
- Gong, L. and Yeh, E.T. (1999) J Biol Chem 274, 12036-42.
- Wada, H. et al. (2000) J Biol Chem 275, 17008-15.
- Sakata, E. et al. (2007) Nat Struct Mol Biol 14, 167-8.
- Kawakami, T. et al. (2001) EMBO J 20, 4003-12.
- Podust, V.N. et al. (2000) Proc Natl Acad Sci USA 97, 4579-84.
- Wu, K. et al. (2002) J Biol Chem 277, 516-27.
- Amir, R.E. et al. (2002) J Biol Chem 277, 23253-9.
- Herrmann, J. et al. (2007) Circ Res 100, 1276-91.
- Walden, H. et al. (2003) Mol Cell 12, 1427-37.
- Walden, H. et al. (2003) Nature 422, 330-4.
- Huang, D.T. et al. (2004) Nat Struct Mol Biol 11, 927-35.
- Soucy, T.A. et al. (2009) Nature 458, 732-6.
- Swords, R.T. et al. (2010) Blood 115, 3796-800.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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