WB
H M R Mk B
Endogenous
52
Rabbit
#Q9P289
51765
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MST4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Mammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple biological processes, including apoptosis, morphogenesis, cell migration, and cytoskeletal rearrangements (1). This group of serine/threonine kinases includes a pair of closely related proteins (MST1, MST2) that are functionally distinct from the more distantly related MST3 and MST4 kinases. All four MST kinases share a conserved amino-terminal kinase domain and carboxy-terminal regulatory and interaction domains (1-3). At least three of these kinases (MST1-3) promote apoptosis and are activated by caspase cleavage followed by nuclear translocation of the active kinase. MST1/2 kinases play a key role in the Hippo signaling pathway, an evolutionarily conserved program that controls organ size by regulating cell proliferation, apoptosis, and stem cell self renewal (4).
Mammalian Sterile 20-like kinase 4 (MST4, STK26, MASK) is a Golgi-localized kinase that is cleaved by caspase-3 in vitro. While its potential role in apoptosis is unclear, research studies indicate that MST4 is involved in MAPK and EGF pathway signaling (5,6). MST4 and the serine/threonine kinase YSK1 (STK25) localize to the Golgi apparatus following association with the Golgi scaffold protein GM130. Binding to GM130 activates MST4 through autophosphorylation at Thr178 (7).
- Dan, I. et al. (2001) Trends Cell Biol 11, 220-30.
- Creasy, C.L. et al. (1996) J Biol Chem 271, 21049-53.
- Lee, K.K. and Yonehara, S. (2002) J Biol Chem 277, 12351-8.
- Zhao, B. et al. (2011) Nat Cell Biol 13, 877-83.
- Dan, I. et al. (2002) J Biol Chem 277, 5929-39.
- Sung, V. et al. (2003) Cancer Res 63, 3356-63.
- Preisinger, C. et al. (2004) J Cell Biol 164, 1009-20.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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