WB, IP, IF-IC
H
Endogenous
> 200
Rabbit
#Q92887
1244
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg260 of human MRP2 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Background
Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family (1,2). The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs (2). MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate (3-5). MRP2 is predominately expressed on the apical membranes in the liver (6-9) and kidney proximal tubules (10). It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes (11-13).
- Keppler, D. and Konig, J. (1997) FASEB J 11, 509-16.
- Borst, P. et al. (2000) J Natl Cancer Inst 92, 1295-302.
- Taniguchi, K. et al. (1996) Cancer Res 56, 4124-9.
- Hooijberg, J.H. et al. (1999) Cancer Res 59, 2532-5.
- Cui, Y. et al. (1999) Mol Pharmacol 55, 929-37.
- Büchler, M. et al. (1996) J Biol Chem 271, 15091-8.
- Paulusma, C.C. et al. (1996) Science 271, 1126-8.
- Mayer, R. et al. (1995) J Cell Biol 131, 137-50.
- Ito, K. et al. (1998) J Biol Chem 273, 1684-8.
- Schaub, T.P. et al. (1997) J Am Soc Nephrol 8, 1213-21.
- Dubin, I.N. and Johnson, F.B. (1954) Medicine (Baltimore) 33, 155-97.
- Kartenbeck, J. et al. (1996) Hepatology 23, 1061-6.
- Paulusma, C.C. et al. (1997) Hepatology 25, 1539-42.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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