| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Iba1/AIF-1 (E4O4W) XP® Rabbit mAb | 17198 | 20 µl | 17 kDa | Rabbit IgG |
| TMEM119 (E3E1O) Rabbit mAb | 90840 | 20 µl | Rabbit IgG | |
| ASC/TMS1 (D2W8U) Rabbit mAb | 67824 | 20 µl | 22 kDa | Rabbit IgG |
| HS1 (D5A9) XP® Rabbit mAb | 3892 | 20 µl | 80 kDa | Rabbit IgG |
| CD11b/ITGAM (M1/70) Rat mAb | 46512 | 20 µl | Rat IgG2b kappa | |
| CD45 (30-F11) Rat mAb | 55307 | 20 µl | Rat IgG2b kappa | |
| F4/80 (D4C8V) XP® Rabbit mAb | 30325 | 20 µl | 65-250 kDa | Rabbit IgG |
| Ki-67 (D3B5) Rabbit mAb | 9129 | 20 µl | Rabbit IgG | |
| CD68 (E3O7V) Rabbit mAb | 97778 | 20 µl | 70-80, 130-140, 200 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Mouse Microglia Marker IF Antibody Sampler Kit provides an economical means of detecting proteins identified as microglia markers by immunofluorescence and/or western blot. This kit includes enough primary antibodies to perform at least twenty IF-F tests or two western blot experiments per primary antibody.
Storage
Background
Microglia are the resident macrophages of the central nervous system, responsible for immune response and maintenance of CNS homeostasis (1). Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (2,3). Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (4). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (5). TMEM119 and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM) (6). Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (7). F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (8-10). Expression of F4/80 has been observed in microglia and subset populations of dendritic cells (11). The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein expressed in all nucleated hematopoietic cells (12). Studies suggest CD45 plays a role in regulation of microglial activation (13,14). CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (15,16). It localizes to the lysosomal membrane and is upregulated during microglial activation (17,18). Ki-67 is a nuclear nonhistone protein (19) universally expressed among proliferating cells and absent in quiescent cells (20). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 and ASC/TMS1 to be useful and specific tools to study microglia (21). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (22) and ASC/TMS1 has been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (23).
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- Bennett, M.L. et al. (2016) Proc Natl Acad Sci U S A 113, E1738-46.
- Satoh, J. et al. (2016) Neuropathology 36, 39-49.
- Deczkowska, A. et al. (2018) Cell 173, 1073-81.
- Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
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- Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15.
- McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9.
- Greter, M. et al. (2015) Front Immunol 6, 249.
- Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74.
- Tan, J. et al. (2000) J Biol Chem 275, 37224-31.
- Cosenza-Nashat, M.A. et al. (2006) Brain Pathol 16, 256-65.
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- Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
- Wong, A.M. et al. (2005) Neurosci Lett 390, 76-80.
- Hendrickx, D.A.E. et al. (2017) J Neuroimmunol 309, 12-22.
- Mincheva, A. et al. (1994) Cytogenet Cell Genet 65, 276-7.
- Doorn, K.J. et al. (2014) Neural Plast 2014, 959154.
- Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
- Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
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Background References
Trademarks and Patents
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