Mouse IL-3 Neutralizing (D6C1) Rabbit mAb (#12222) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

REACTIVITY:

SENSITIVITY:

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P08700

Entrez-Gene Id:

3562

Product Information

Product Usage Information

CST recommends incubation of the neutralizing antibody with the intended target for 1 hr at 37ºC before addition to the experiment at an optimal concentration determined by the user.

Reconstitution:

Add sterile 10 mM HEPES pH 7.0 to a final concentration of greater than 50 μg/ml. Solubilize for 20 min at room temperature with occasional gentle vortexing.

Application	Dilution
Neutralizing	1:1

Formulation

Lyophilized from a 0.2 µm filtered solution in 10 mM HEPES with trehalose.

Storage

Store lyophilized material at -20ºC. After reconstitution, recommended storage at 4ºC for 1 month or -20ºC for 6 months. Avoid repeated freeze/thawing.

Specificity / Sensitivity

Mouse IL-3 Neutralizing (D6C1) Rabbit mAb binds to mouse IL-3 and neutralizes its effects in a BaF3 cell proliferation assay. This antibody does not cross-react with human IL-3, human IL-5, human GM-CSF, or mouse GM-CSF.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant mouse IL-3 protein.

Product Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse IL-3 Neutralizing (D6C1) Rabbit mAb has been shown to neutralize the proliferation of IL-3-dependent BaF3 cells in vitro with an ND₅₀ in the range of 0.5-3.5 µg/ml.

Endotoxin	<0.1 EU/µg of antibody

Background

IL-3 is produced by T cells, mast cells, and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid, and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support the culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3-specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2 and the PI3K/Akt pathway, and phosphorylation of Stat1/3/5/6 (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3-5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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