WB, IP
H
Endogenous
55
Rabbit IgG
#P50219
3110
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser231 of human MNX1/HB9 protein.
Background
Motor neuron and pancreas homeobox protein 1 (MNX1 or Homeobox protein HB9) is a homeobox transcription factor that has conserved functions in motor neuron differentiation and pancreas development. The mammalian central nervous system (CNS) is composed of specialized neurons, and the specification of neuronal identity in the CNS is determined early in development by spatially-specified expression of cell-intrinsic determinants, many of which are transcription factors (1). MNX1/HB9 is one such transcription factor. In response to developmental signals, MNX1/HB9 is expressed transiently to drive differentiation of ventral progenitor cells into somatic motor neurons (2,3). Mutations in the gene encoding human MNX1/HB9 are linked to Currarino syndrome, a developmental disease caused by dorsal-ventral patterning defects resulting in presacral tumors, sacral agenesis, and anorectal malformation in patients suffering from this disease (4,5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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