WB, IP
H
Endogenous
130
Rabbit IgG
#Q8TDZ2
64780
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1038 of human MICAL1 protein.
Background
Molecule interacting with CasL protein 1 (MICAL1) is a Protein-methionine sulfoxide oxidase. MICAL1 can bind directly to F-actin and oxidize specific methionine residues to promote actin filament disassembly (1-3). MICAL1 is an important component of semaphorin signaling cascades that has effects on cell movement, angiogenesis, immunology, diabetes, and cancer (2,4-6). MICAL1 binds to NDR1/2 and antagonizes MST1-induced NDR activation and apoptosis (7).
- Hung, R.J. et al. (2013) Nat Cell Biol 15, 1445-54.
- Lee, B.C. et al. (2013) Mol Cell 51, 397-404.
- Giridharan, S.S. and Caplan, S. (2014) Antioxid Redox Signal 20, 2059-73.
- Terman, J.R. et al. (2002) Cell 109, 887-900.
- Aggarwal, P.K. et al. (2015) Diabetes 64, 1743-59.
- Loria, R. et al. (2015) Oncotarget 6, 2779-93.
- Zhou, Y. et al. (2011) Mol Cell Biol 31, 3603-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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