WB
H M R
Endogenous
80
Rabbit IgG
#Q5U5Q3
51320
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala54 of human MEX3C protein.
Background
MEX3C is an RNA-binding E3 ubiquitin ligase implicated in a diverse set of biological functions.
Along with PIGN and ZNF516, MEX3C has been identified as a suppressor of chromosome instability (CIN), which drives intratumor heterogeneity and has been associated with poor prognosis in colon cancer. Silencing of the Mex3c gene induces chromosome instability through replication stress/impaired replication fork progression (1).
In mice, absence of the Mex3c gene causes growth retardation and reduced insulin-like growth factor 1 (IGF1) expression (2). Researchers have shown that murine MEX3C plays a role in controlling energy expenditure, physical activity and degree of adiposity (3,4). In the immune system, MEX3C is involved in post-transcriptional regulation of HLA-A2 (5), and plays a role in eliciting antiviral response (6).
- Burrell, R.A. et al. (2013) Nature 494, 492-6.
- Jiao, Y. et al. (2012) Mol Biol Cell 23, 1404-13.
- Jiao, Y. et al. (2012) Mol Cell Biol 32, 4350-62.
- Han, C. et al. (2014) J Endocrinol 221, 457-68.
- Cano, F. et al. (2012) EMBO J 31, 3596-606.
- Kuniyoshi, K. et al. (2014) Proc Natl Acad Sci U S A 111, 5646-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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