WB, IF-IC
H M R Mk
Endogenous
78
Rabbit IgG
#Q86W50
79066
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala9 of human METTL16 protein.
Background
METTL16 is an N6-adenosine methyltransferase responsible for the regulation of the MAT2A gene, which encodes S-adenosylmethionine (SAM) synthase. Upon SAM depletion, MAT2A expression increases due to a splicing event of a retained intron. Alternative splicing and mRNA stability is governed by adenosine methylation in the 3’ UTR of the MAT2A mRNA by METTL16. These marks are then read by YTHDC1, and knockdown of either METTL16 or YTHDC1 results in decreased response to lack of SAM (1,2). The METTL16 methyltransferase domain differs from METTL3 and METTL14 as it contains an extra N-terminal module, suggesting a different set of target mRNAs (3,4). Lack of METTL16 during development has been shown to be embryonically lethal, resulting in a dysregulated transcriptome that cannot proceed past the 64-cell blastocyst stage (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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