WB, IHC-Bond, IHC-P
M
Endogenous
20-40
Rabbit IgG
#P35461
546644
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:300 - 1:1200 |
| Immunohistochemistry (Paraffin) | 1:75 - 1:300 |
Storage
For a carrier free (BSA and azide free) version of this product see product #75028.
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with mouse Ly-6G recombinant protein.
Background
The Ly-6 complex is a series of genes found on chromosome 15. These genes code for a number of different proteins that can be used as surface markers. The family members vary in their biologic expression and have been shown to be involved in cell signaling and cell adhesion (1). The structure of these proteins includes a motif known as the LU domain that has three loops comprised of disulfide bonds. These bonds are formed by 8 to 10 cysteines that can cause differences in the length of the loops as well as the sequences at each tip (2,4). There are 11 known Ly-6 genes on murine chromosome 15 that code for different proteins. These family members, excluding secreted Ly6/Plaur domain containing 1 coded by the Slurp1 gene, are attached to the cell surface by a GPI anchor near the C terminus. The structure of these proteins may play a role in transmembrane interactions, and downstream signaling cascades (1,2).
Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5).
Ly-6G is used as a marker for mouse neutrophils (2,3). It is also expressed by mouse polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) (6).
- Bamezai, A. (2004) Arch Immunol Ther Exp (Warsz) 52, 255-66.
- Lee, P.Y. et al. (2013) J Leukoc Biol 94, 585-94.
- Fleming, T.J. et al. (1993) J Immunol 151, 2399-408.
- Tsetlin, V. (1999) Eur J Biochem 264, 281-6.
- Pflugh, D.L. et al. (2000) J Immunol 165, 313-21.
- Bronte, V. et al. (2016) Nat Commun 7, 12150.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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