WB, IP, IHC-P
H Mk
Endogenous
65
Rabbit IgG
#Q86X29
51599
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly449 of human LSR protein.
Background
The lipolysis-stimulated lipoprotein receptor (LSR, LISCH) is an immunoglobulin superfamily member and single pass transmembrane protein that binds the apolipoprotein B (ApoB) and apolipoprotein E (ApoE) lipoproteins (1). LSR is responsible for the cellular uptake of triacylglyceride-rich lipoproteins and supports lipid distribution between the liver and peripheral tissues (1,2). The LSR protein is expressed at the cell membrane as a heterodimer consisting of α and β subunits, which are produced as alternative splice variants from a single gene (3). Research studies suggest that LSR acts as the host cell surface receptor for multiple Clostridium toxins (4) and participates in the formation of tricellular tight junctions in epithelial cells (5). Additional studies demonstrate that LSR expression is up-regulated in several cancer types, including breast, bladder, and colorectal cancer, which could lead to pro-tumorigenic changes in metabolism (6-8).
- Bihain, B.E. and Yen, F.T. (1992) Biochemistry 31, 4628-36.
- Yen, F.T. et al. (1994) Biochemistry 33, 1172-80.
- Yen, F.T. et al. (1999) J Biol Chem 274, 13390-8.
- Papatheodorou, P. et al. (2012) Infect Immun 80, 1418-23.
- Masuda, S. et al. (2011) J Cell Sci 124, 548-55.
- García, J.M. et al. (2007) Clin Cancer Res 13, 6351-8.
- Herbsleb, M. et al. (2008) BMC Med Genomics 1, 31.
- Reaves, D.K. et al. (2014) PLoS One 9, e91747.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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