WB
H M R
Endogenous
98
Rabbit
#Q96FE5
84894
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu400 of human LINGO-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Leucine-rich repeat and immunoglobulin domain-containing protein 1 (LINGO-1) is a potent negative modulator of neuronal processes, including neuronal survival, axonal integrity, oligodendrocyte differentiation, and myelination (1-5). LINGO-1, Nogo receptor (NgR), and p75 neurotrophin receptor (p75NTR), or TNF receptor orphan Y (TROY) form a tripartite receptor complex, which activates RhoA/ROCK signaling and is responsible for the inhibition effect of myelin- associated factors (6,7). LINGO-1 is abundantly expressed in the brain and is implicated in various neurodegenerative disorders, such as Essential tremor, multiple sclerosis, and Parkinson’s disease (8-11). Recently, LINGO-1 was reported to bind directly to amyloid precursor protein (APP), promoting its degradation through lysosomal proteolysis (12). This research study implicated that LINGO-1 plays a critical role in the pathophysiology of Alzheimer's disease.
- Shao, Z. et al. (2005) Neuron 45, 353-9.
- Mi, S. et al. (2005) Nat Neurosci 8, 745-51.
- Zhang, Z. et al. (2009) J Biol Chem 284, 15717-28.
- Jepson, S. et al. (2012) J Biol Chem 287, 22184-95.
- Lee, X. et al. (2014) Mol Cell Neurosci 60, 36-42.
- Mi, S. et al. (2004) Nat Neurosci 7, 221-8.
- Park, J.B. et al. (2005) Neuron 45, 345-51.
- Inoue, H. et al. (2007) Proc Natl Acad Sci U S A 104, 14430-5.
- Delay, C. et al. (2014) Mov Disord 29, 1637-47.
- Oh, J. and Calabresi, P.A. (2013) Lancet Neurol 12, 1115-26.
- Andrews, J.L. and Fernandez-Enright, F. (2015) Neurosci Biobehav Rev 56, 97-114.
- de Laat, R. et al. (2015) Pathobiol Aging Age Relat Dis 5, 25796.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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