Leupaxin (D4I3B) Rabbit mAb (#59309) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, W-S, IP

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#O60711

Entrez-Gene Id:

9404

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Leupaxin (D4I3B) Rabbit mAb recognizes endogenous levels of total leupaxin protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg35 of human leupaxin protein.

Background

Leupaxin is a member of the paxillin family of scaffold proteins whose functions include regulation of cell adhesion, migration and metastasis (1). Leupaxin suppresses integrin-dependent tyrosine phosphorylation of paxillin and affects the regulation of cell-matrix adhesion (2). Researchers have implicated leupaxin in the progression of human hepatocellular carcinoma through its regulation of beta-catenin-mediated transcription (3). Researchers have also shown that leupaxin regulates actin dynamics and migration of prostate cancer cells through its interaction with caldesmon (4) and down-regulation of p120-catenin (5). Studies in breast cancer implicate leupaxin in the regulation of ER-alpha transcription and invasiveness (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

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