WB, IP, IF-IC, FC-FP
H
Endogenous
40-50
Rabbit IgG
#Q9UJ71
50489
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:3200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #49830.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human Langerin protein.
Background
Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin including Langerhans cells in the epidermis and langerin+/CD103+ dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8).
- Valladeau, J. et al. (2000) Immunity 12, 71-81.
- Poulin, L.F. et al. (2007) J Exp Med 204, 3119-31.
- Ginhoux, F. et al. (2007) J Exp Med 204, 3133-46.
- Bursch, L.S. et al. (2007) J Exp Med 204, 3147-56.
- Kissenpfennig, A. et al. (2005) Mol Cell Biol 25, 88-99.
- Stambach, N.S. and Taylor, M.E. (2003) Glycobiology 13, 401-10.
- Idoyaga, J. et al. (2008) J Immunol 180, 3647-50.
- Igyártó, B.Z. et al. (2011) Immunity 35, 260-72.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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