WB, IP
H M R
Endogenous
220-250
Rabbit IgG
#P11047
3915
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1365 of human LAMC1 protein.
Background
Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of alpha (LAMA), beta (LAMB), and gamma (LAMC) chains. LAMC1 (laminin gamma-1) is a ubiquitously expressed gamma chain, and has three distinct functional domain structures. The N-terminal LN domain binds to specific alpha and beta chains to form a unique laminin heterotrimer. The LE domain mediates interactions with nidogens, while the C-terminal coil-coil fragment is necessary for agrin association. Collectively, these interactions are critical for stabilization and function of the BM (1). Genetic ablation of LAMC1 has demonstrated a critical role for this protein in embryo implantation, lung and kidney development, and neuronal Schwann cell myelination and regeneration, in addition to Trypanosome infection (2-5). Upregulation of LAMC1 has also been associated with tumor progression in multiple tumor types (6-9), possibly by creating a BM environment that is favorable for cancer cell metastasis and invasion.
- Yurchenco, P.D. (2011) Cold Spring Harb Perspect Biol 3, a004911.
- Ramathal, C. et al. (2011) J Biol Chem 286, 19860-71.
- Willem, M. et al. (2002) Development 129, 2711-22.
- Chen, Z.L. and Strickland, S. (2003) J Cell Biol 163, 889-99.
- Nde, P.N. et al. (2012) Front Immunol 3, 337.
- Liétard, J. et al. (1997) Am J Pathol 151, 1663-72.
- Nishikawa, R. et al. (2014) Int J Oncol 45, 401-10.
- Huang, S.X. et al. (2016) Int J Oncol 49, 217-24.
- Kashima, H. et al. (2015) Gynecol Oncol 139, 338-44.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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