Revision 1

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Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

77

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q9BQL6

Entrez-Gene Id:

55612

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Specificity / Sensitivity

Kindlin-1 (D1K4C) Rabbit mAb recognizes endogenous levels of total Kindlin-1 protein. This antibody does not cross-react with Kindlin-2 or Kindlin-3.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human Kindlin-1 protein.

Background

The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3).

  1. Ye, F. and Petrich, B.G. (2011) Curr Opin Hematol 18, 356-60.
  2. Meves, A. et al. (2009) Trends Cell Biol 19, 504-13.
  3. Karaköse, E. et al. (2010) J Cell Sci 123, 2353-6.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 1
#36734

Kindlin-1 (D1K4C) Rabbit mAb

Western Blotting Image 1: Kindlin-1 (D1K4C) Rabbit mAb Expand Image
使用 Kindlin-1 (D1K4C) Rabbit mAb(上)或 Myc-Tag (71D10) Rabbit mAb #2278(下)时,对转染空载 (-) 或转染 Myc/DDK 标记的全长人 kindlin-1 蛋白 (hKindlin-1-Myc/DDK;+)、kindlin-2 蛋白 (hKindlin-2-Myc/DDK;+)或 kindlin-3 蛋白 (hKindlin-3-Myc/DDK;+)表达载体的293T 细胞提取物进行蛋白质印迹分析。
Western Blotting Image 2: Kindlin-1 (D1K4C) Rabbit mAb Expand Image
使用 Kindlin-1 (D1K4C) Rabbit mAb(上)或 α-Actinin (D6F6) Rabbit mAb #6487(下)对不同细胞系的提取物进行蛋白质印迹分析。在泳道 2 中,将 COLO 205 提取物用牛小肠磷酸酶 (CIP) 和 λ 磷酸酶处理,显示 COLO 205提取物中的高分子量条带是磷酸化的 kindlin-1 蛋白。
Immunoprecipitation Image 1: Kindlin-1 (D1K4C) Rabbit mAb Expand Image
从 COLO 205 细胞提取物免疫沉淀 kindlin-1。泳道 1 是 10 % input,泳道 2 是 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900,并且泳道 3 是 Kindlin-1 (D1K4C) Rabbit mAb。使用 Kindlin-1 (D1K4C) Rabbit mAb 进行蛋白质印迹分析。