WB, IP
H Mk
Endogenous
135
Rabbit
#Q8IX03
23286
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala585 of human KIBRA protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of YAP (Yes-associated protein), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). Recent studies have identified KIBRA as a novel regulator of Hippo signaling (5-7). KIBRA has been shown to regulate Hippo signaling through its interaction with tumor suppressors Merlin (Mer) and Expanded (Ex) in Drosophila (7) and by associating with large tumor suppressors LATS1 and LATS2 in humans (8). In humans, KIBRA is predominantly expressed in the kidney and brain (9) and has been shown to play a role in hippocampus-related memory performance (10-12). Recent studies have shown that phosphorylation of KIBRA is highest during mitosis and is controlled by aurora kinase and protein phosphatase 1 (13).
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- Harvey, K.F. et al. (2003) Cell 114, 457-67.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46.
- Baumgartner, R. et al. (2010) Dev Cell 18, 309-16.
- Genevet, A. et al. (2010) Dev Cell 18, 300-8.
- Yu, J. et al. (2010) Dev Cell 18, 288-99.
- Xiao, L. et al. (2011) J Biol Chem 286, 7788-96.
- Kremerskothen, J. et al. (2003) Biochem Biophys Res Commun 300, 862-7.
- Papassotiropoulos, A. et al. (2006) Science 314, 475-8.
- Bates, T.C. et al. (2009) Neurosci Lett 458, 140-3.
- Schaper, K. et al. (2008) Neurobiol Aging 29, 1123-5.
- Xiao, L. et al. (2011) J Biol Chem 286, 36304-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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