IP, FC-FP
H
Endogenous
150: alpha9, 130: beta1
Mouse IgG1
#P05556, #Q13797
3688, 3680
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with murine L cells transfected with human α9 integrin protein.
Background
Integrins are transmembrane glycoproteins that form heterodimers consisting of one α and one β subunit. The dimers act as receptors for extracellular matrix (ECM) proteins at sites of cell adhesion, and interact with focal adhesion (FA) proteins on the cytosolic side, forming the connection between the ECM and the actin cytoskeleton. Signaling to and from integrins regulates cell adhesion, motility, proliferation, apoptosis and gene expression, impacting cellular processes such as development, wound healing, immune response, invasion, metastasis and angiogenesis (reviewed in 1,2). α9β1 integrin is expressed in epithelial cells, smooth and skeletal muscle, neutrophils and hepatocytes (3). Its ligands include the ECM protein tenascin (4) and vascular cell adhesion molecule-1 (VCAM-1) (5). The cytoplasmic domain of α9 integrin binds the focal adhesion adaptor protein, paxillin, inhibiting cell spreading (6,7). Binding of the α9 cytoplasmic domain to spermidine/spermine N(1)-acetyltransferase (SSAT) mediates α9/β1 enhancement of cell migration (8). Physiological functions include development of the lymphatic system (9), possibly through binding to the lymphatic vascular endothelial growth factors VEGF-C and -D (10), neutrophil migration (5), and myogenic differentiation (11).
- Calderwood, D.A. et al. (2000) J Biol Chem 275, 22607-10.
- ffrench-Constant, C. and Colognato, H. (2004) Trends Cell. Biol. 14, 678-686.
- Palmer, E.L. et al. (1993) J. Cell Biol. 123, 1289-1297.
- Yokosaki, Y. et al. (1994) J. Biol. Chem. 269, 26691-26696.
- Taooka, Y. et al. (1999) J. Cell Biol. 145, 413-420.
- Young, B.A. et al. (2001) Mol. Biol. Cell 12, 3214-3225.
- Liu, S. et al. (2001) J. Biol. Chem. 276, 37086-37092.
- Chen, C. et al. (2004) J. Cell Biol. 167, 161-170.
- Huang, X.Z. et al. (2000) Mol. Cell Biol. 20, 5208-5215.
- Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280, 4544-4552.
- Lafuste, P. et al. (2005) Mol. Biol. Cell 16, 861-870.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
