WB, IP
H
Endogenous
160
Rabbit IgG
#Q9UKX5
22801
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human Integrin α11 protein.
Background
Integrin α11, also known as type I collagen receptor, is a single-pass type I transmembrane protein that mediates cell-matrix adhesion and is part of the family of integrins that play a pivotal role in regulation processes of cell proliferation, differentiation, migration, tumor invasion, and metastasis (1-3). Integrin α11 is highly expressed in embryonic fibroblasts and myofibroblasts and to varying degrees in the cytoplasm of most human tissues (4-6). In non-small cell lung cancers, integrin α11 overexpression correlates with tumorigenicity and formation of secondary metastatic lesions by increasing the migration and invasion capacity (7,8). TGFβ activation of integrin α11 results in significant upregulation and overexpression by cancer associated fibroblasts in the tumor stroma; therefore, it has been used as a prognostic marker in certain cancers (9,10). As a receptor for type I collagen, which is found in fibrotic tissue of the liver, lung, and kidney, integrin α11 colocalizes with α-smooth muscle actin. Type I collagen is the most abundant form in the body, indicating a role for targeting integrin α11 in the therapeutic intervention of fibrotic disease (4).
- Wu, P. et al. (2019) PeerJ 7, e8299.
- Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
- Schnittert, J. et al. (2018) Adv Drug Deliv Rev 129, 37-53.
- Bansal, R. et al. (2017) Exp Mol Med 49, e396.
- Velling, T. et al. (1999) J Biol Chem 274, 25735-42.
- Lehnert, K. et al. (1999) Genomics 60, 179-87.
- Ando, T. et al. (2020) Cancer Sci 111, 200-208.
- Lu, N. et al. (2010) Matrix Biol 29, 166-76.
- Schnittert, J. et al. (2019) FASEB J 33, 6609-6621.
- Parajuli, H. et al. (2017) J Oral Pathol Med 46, 267-275.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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