WB
H M Mk
Endogenous
70
Rabbit IgG
#O00425
10643
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly572 of human IMP3 protein.
Background
Insulin-like growth factor-II mRNA-binding proteins (IMPs) belong to a family of zipcode-binding proteins (1,2). Three members of this family, IMP1, IMP2, and IMP3, have been identified (1,2). They contain two RNA recognition motifs, four K homology domains, and were found to function in mRNA localization, turnover, and translation control (1,2). Research studies have implicated these proteins in a variety of physiological and pathological processes, such as growth and development (3), testicular neoplasia (4), and melanocytic neoplasia (5).
IMP3 is an oncofetal protein expressed in developing tissues as well as in many tumor types. Expression of IMP3 is a marker for poor prognosis and metastasis in a variety of cancers (6-10).
- Nielsen, F.C. et al. (2002) J Cell Sci 115, 2087-97.
- Nielsen, J. et al. (2003) Biochem J 376, 383-91.
- Hansen, T.V. et al. (2004) Mol Cell Biol 24, 4448-64.
- Hammer, N.A. et al. (2005) Reproduction 130, 203-12.
- Pryor, J.G. et al. (2008) Mod Pathol 21, 431-7.
- Samanta, S. et al. (2016) Oncogene 35, 1111-21.
- Hoffmann, N.E. et al. (2008) Cancer 112, 1471-9.
- Jiang, Z. et al. (2008) Cancer 112, 2676-82.
- Köbel, M. et al. (2009) Mod Pathol 22, 469-75.
- Jeng, Y.M. et al. (2008) Hepatology 48, 1118-27.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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