WB
H
Endogenous
30
Rabbit IgG
#O95760
90865
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human IL-33 recombinant protein and reacts with an epitope surrounding Glu165.
Background
IL-33 (Interleukin-33, NF-HEV) is a nuclear cytokine that is part of the IL-1 family (1,2). Originally discovered as the ‘nuclear factor from high endothelial venules,’ it is widely expressed by endothelial cells as well as epithelial barrier tissues (1,5). IL-33 is involved in regulating type 2-associated innate immune responses (2,4). It is the ligand for ST2 which, along with IL-1RAcP, forms the IL-33R signaling complex (2,3). IL-33 functions as an alarmin upon cellular damage or stress, and is associated with allergic inflammation and asthma (5,6).
- Baekkevold, E.S. et al. (2003) Am J Pathol 163, 69-79.
- Schmitz, J. et al. (2005) Immunity 23, 479-90.
- Chackerian, A.A. et al. (2007) J Immunol 179, 2551-5.
- Smithgall, M.D. et al. (2008) Int Immunol 20, 1019-30.
- Moussion, C. et al. (2008) PLoS One 3, e3331.
- Cayrol, C. and Girard, J.P. (2014) Curr Opin Immunol 31, 31-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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