WB, IP
H
Endogenous
60-70
Rabbit IgG
#P27930
7850
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-1R2 protein.
Background
IL-1R2 is a type I transmembrane glycoprotein that is expressed predominantly by cells of the myeloid lineage (1). Like IL-1R1, the extracellular domain of IL-1R2 is capable of binding to all three forms of IL-1 (2). Unlike IL-1R1, however, membrane-bound IL-1R2 has a short cytoplasmic tail and does not transduce signals upon binding IL-1 but rather functions as a decoy receptor to dampen IL-1 signaling (3,4). As a negative regulator of the IL-1 receptor signaling complex, research studies have also demonstrated that membrane-bound IL-1R2 undergoes regulated intramembrane proteolysis in response to pro-inflammatory molecules. This process converts the extracellular domain of IL-1R2 into a soluble decoy receptor capable of binding IL-1, limiting its capacity to elicit productive signals through membrane-bound IL-1R1 (5,6). Recent studies have demonstrated that some types of human cancers are enriched with regulatory T cells that express high levels of IL-1R2, an attribute that may contribute to their role in immunosuppression (7,8).
- Colotta, F. et al. (1996) J Immunol 156, 2534-41.
- McMahan, C.J. et al. (1991) EMBO J 10, 2821-32.
- Neumann, D. et al. (2000) J Immunol 165, 3350-7.
- Colotta, F. et al. (1993) Science 261, 472-5.
- Orlando, S. et al. (1997) J Biol Chem 272, 31764-9.
- Uchikawa, S. et al. (2015) Cytokine 71, 238-45.
- Plitas, G. et al. (2016) Immunity 45, 1122-1134.
- De Simone, M. et al. (2016) Immunity 45, 1135-1147.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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