WB
H
Endogenous
32
Rabbit IgG
#P24593
3488
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro139 of human IGFBP5 protein.
Background
Insulin-like growth factor (IGF) signaling plays a major role in regulating the proliferation and metabolism of normal and malignant cells. IGF-binding proteins (IGFBPs) play an integral role in modifying IGF actions in a wide variety of cell types. The six IGFBP family members share a high affinity for IGF binding and are structurally related, but are encoded by distinct genes (1). IGFBPs can exert stimulatory or inhibitory effects by controlling IGF availability through high affinity binding of IGF at the carboxy-terminal domain (2,3). IGFBP5 belongs to the high affinity IGF binding family. The effects of IGFBP5 on cancer development are either positive or negative depending on the cancer type (4). IGFBP5 has been shown to regulate tumor cell survival, apoptosis, migration, and metastasis by mechanism of IGF-dependent or IGF-independent actions (4-6). Downregulation of IGFBP5 is associated with therapeutic resistance in breast cancer and esophageal carcinoma. Meanwhile, upregulation of the protein can reverse drug resistance (7-9).
- Hwa, V. et al. (1999) Endocr Rev 20, 761-87.
- Yu, H. and Rohan, T. (2000) J Natl Cancer Inst 92, 1472-89.
- Martin, J.L. and Baxter, R.C. (2011) Growth Factors 29, 235-44.
- Güllü, G. et al. (2012) Chin J Cancer 31, 266-80.
- Hwang, J.R. et al. (2016) Sci Rep 6, 39334.
- Ding, M. et al. (2016) Nat Cell Biol 18, 319-27.
- Chan, D. et al. (2018) Cells 7, pii: E143. doi: 10.3390/cells7100143.
- Wang, W. et al. (2018) Mol Cancer Ther 17, 1973-83.
- Leyh, B. et al. (2015) Oncotarget 6, 39307-28.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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