WB, IHC-P
H
Endogenous
30
Rabbit IgG
#P08833
3484
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro240 of human IGFBP1 protein.
Background
Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. There are six known IGFBP family members (IGFBP1-6), which are structurally related, but encoded by distinct genes. IGFBPs have high affinity for IGFs; in some contexts, IGFBPs inhibit IGF actions by preventing access to IGF receptors, while in others they potentiate IGF actions by facilitating ligand-receptor interaction (1-3). IGFBP1 is produced primarily by the liver and secreted into circulation, and studies show its expression can be negatively regulated by insulin (4, 5). Notably, low levels of IGFBP1 were shown to predict the future onset of Type 2 diabetes (5). Reduced expression of IGFBP1 expression was also associated with tumor progression in breast cancer, prostate cancer, pancreatic cancer and colorectal cancer, possibly stemming from reduced inhibition of mitogenic IGF signaling (6-9). Notably however, other research studies have reported increased levels of IGFBP1 in selected tumor types; in human schwannoma, increased IGFBP1 was associated with stimulation of the integrin β1/FAK pathway, supporting the concept of IGF-independent signaling functions for selected IGFBPs (10,11).
- Duan, C. (2002) J Endocrinol 175, 41-54.
- Sandhu, M.S. et al. (2002) J Natl Cancer Inst 94, 972-80.
- Baxter, R.C. (2014) Nat Rev Cancer 14, 329-41.
- Ross, R.J. et al. (1994) J Endocrinol 141, 377-82.
- Lewitt, M.S. et al. (2014) J Clin Med 3, 1561-74.
- Park, J.H. et al. (2015) Neoplasia 17, 421-33.
- Wolpin, B.M. et al. (2007) Cancer Res 67, 7923-8.
- Cao, Y. et al. (2015) Int J Cancer 136, 2418-26.
- Purandare, S.M. et al. (2009) Am J Med Genet A 149A, 1740-8.
- Ammoun, S. et al. (2012) Oncogene 31, 1710-22.
- Wheatcroft, S.B. et al. (2009) Trends Endocrinol Metab 20, 153-162.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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