WB, IP
H M Mk
Endogenous
15
Rabbit IgG
#Q02363
3398
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn97 of human Id2 protein.
Background
Inhibitor of DNA-binding-2 (Id2) is a member of the Id proteins which belong to the helix-loop-helix (HLH) protein family. The Id protein functions by binding to specific transcription factors and preventing their dimerization and DNA binding (1-3). Id2 interacts with a wide variety of transcription factors including E proteins (5), TCS (4), Pax (3) and the tumor suppressor Rb (1). Id2 has been shown to be important in regulating cellular differentiation, proliferation, development and tumorgenesis (6-8). In tumor cells, increased levels of Id2 functionally inactivate Rb, leading to cellular transformation and cancer (9,10). Id2 is therefore a promising therapeutic target for treatment of cancer (11).
- Iavarone, A. et al. (1994) Genes Dev 8, 1270-84.
- Sun, X.H. et al. (1991) Mol Cell Biol 11, 5603-11.
- Roberts, E.C. et al. (2001) Mol Cell Biol 21, 524-33.
- Yates, P.R. et al. (1999) EMBO J 18, 968-76.
- Engel, I. and Murre, C. (2001) Nat Rev Immunol 1, 193-9.
- Benezra, R. et al. (2001) Oncogene 20, 8334-41.
- Lasorella, A. et al. (2001) Oncogene 20, 8326-33.
- Zebedee, Z. and Hara, E. (2001) Oncogene 20, 8317-25.
- Gabellini, C. et al. (2006) Oncogene 25, 5326-32.
- Perk, J. et al. (2005) Nat Rev Cancer 5, 603-14.
- Gray, M.J. et al. (2008) Oncogene 27, 7192-200.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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