Hydroxyproline Antibody (#73812) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-P

REACTIVITY:

All

SENSITIVITY:

Endogenous

MW (kDa):

SOURCE:

Rabbit

Product Information

Product Usage Information

Application	Dilution
Immunohistochemistry (Paraffin)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Hydroxyproline Antibody recognizes endogenous levels of proline only when the proline is hydroxylated. The antibody does not react with non-hydroxylated proline.

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with an antigen containing hydroxyproline. Antibodies are purified by affinity chromatography.

Product Description

Hydroxyproline Antibody provides a means of visualizing hydroxyproline in paraffin-embedded tissues. Using Hydroxyproline Antibody in the IHC assay, global collagen in tissue can be detected, while also providing contextual information that is lost in traditional hydroxyproline assays. Additionally, Hydroxyproline Antibody can be combined with other antibodies to enable detection of other markers of interest.

Background

Collagens are a large family of proteins and collectively they are the most abundant protein in mammals. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (1). Collagen chains have important features that allow helix to form: every third amino acid in the sequence is a glycine, and the chains are co- and post-translationally modified. Prolines are hydroxylated by collagen prolyl 4-hydroxylases. These hydroxyproline modifications are critical for the stability of the triple helix to form fibrils (2).

While proline hydroxylation occurs on a small number of other proteins, the hydroxyproline content of tissue comes almost entirely from collagen, such that hydroxyproline quantitative biochemical assays are routinely utilized as a measurement of collagen (3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

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