WB, IP
H
Endogenous
120
Rabbit IgG
#Q96EP0
55072
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HOIP/RNF31 protein.
Background
HOIL-1-interacting protein (HOIP/RNF31), a RING-type E3 ubiquitin ligase, is the catalytic subunit of the Linear Ubiquitin Chain Assembly Complex (LUBAC) that is associated with TNF-R1 (1). Research studies have shown that the LUBAC consists of three subunits: HOIP, HOIL-1L, and Sharpin that facilitate canonical NF-kB activation in response to pro inflammatory cytokines through M1-linked linear ubiquitination of NEMO and RIP1 (2-6). As part of the LUBAC, HOIP has also been implicated in the negative regulation of interferon-mediated antiviral signaling through the suppression of RIG-I activation (7). The role of HOIP in LUBAC function and human disease is underscored by naturally occurring mutations in HOIP that impair LUBAC assembly and NF-kb activation. Patients that are homozygous for this mutation in HOIP have multi organ auto inflammation and immunodeficiency (8).
- Haas, T.L. et al. (2009) Mol Cell 36, 831-44.
- Smit, J.J. et al. (2012) EMBO J 31, 3833-44.
- Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32.
- Tokunaga, F. et al. (2011) Nature 471, 633-6.
- Blackwell, K. et al. (2013) Mol Cell Biol 33, 1901-15.
- Ikeda, F. et al. (2011) Nature 471, 637-41.
- Inn, K.S. et al. (2011) Mol Cell 41, 354-65.
- Boisson, B. et al. (2015) J Exp Med 212, 939-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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