WB, IP
H M R
Endogenous
18
Rabbit
#P52926
8091
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human HMGA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (2,4-6). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (7-10).
- Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
- Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
- Monzen, K. et al. (2008) Nat Cell Biol 10, 567-74.
- Nishino, J. et al. (2008) Cell 135, 227-39.
- Li, O. et al. (2006) Genesis 44, 523-9.
- Li, O. et al. (2007) FEBS Lett 581, 3533-7.
- Fusco, A. and Fedele, M. (2007) Nat Rev Cancer 7, 899-910.
- Rawlinson, N.J. et al. (2008) Cancer Genet Cytogenet 181, 119-24.
- Wei, J.J. et al. (2010) Am J Surg Pathol 34, 18-26.
- Mahajan, A. et al. (2010) Mod Pathol 23, 673-81.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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