WB, IP, IF-IC, FC-FP
H Mk
Endogenous
60
Rabbit IgG
#O94992
10614
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein.
Background
Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).
- Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
- Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
- Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
- Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
- Wada, T. et al. (1998) EMBO J 17, 7395-403.
- Yamada, T. et al. (2006) Mol Cell 21, 227-37.
- Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
- Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
- Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
- Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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