WB, IHC-P
H Mk
Endogenous
60
Rabbit IgG
#P06744
2821
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:1000 - 1:4000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro8 of human GPI protein.
Background
Glucose-6-phosphate isomerase (GPI) is a multi-functional protein belonging to the glucose phosphate isomerase family (1,2). As an intracellular metabolic enzyme, GPI plays a pivotal role in glycolysis and gluconeogenesis by catalyzing the interconversion of D-glucose-6-phosphate and D-fructose-6-phosphate (3). GPI is also secreted, where it functions as a cytokine (referred to as Autocrine Motility Factor, AMF), acting via the E3-ubiquitin-protein ligase AMFR/gp78 (4). In normal tissues, GPI/AMF has been shown to promote both immune cell maturation and neuronal cell survival (5,6). It is also secreted in abundance by some tumor cells (7), where it has been shown to promote tumor cell migration and metastasis (8,9).
- Haga, A. et al. (2000) Biochim Biophys Acta 1480, 235-44.
- Jeffery, C.J. et al. (2000) Biochemistry 39, 955-64.
- Kim, J.W. and Dang, C.V. (2005) Trends Biochem Sci 30, 142-50.
- Fairbank, M. et al. (2009) Mol Biosyst 5, 793-801.
- Gurney, M.E. et al. (1986) Science 234, 574-81.
- Gurney, M.E. et al. (1986) Science 234, 566-74.
- Lucarelli, G. et al. (2015) Medicine (Baltimore) 94, e2117.
- Liotta, L.A. et al. (1986) Proc Natl Acad Sci U S A 83, 3302-6.
- Funasaka, T. and Raz, A. (2007) Cancer Metastasis Rev 26, 725-35.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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