Revision 1

#24642Store at -20C

1 个试剂盒

(5 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
GATA-1 (D52H6) XP® Rabbit mAb 3535 20 µl 43 kDa Rabbit IgG
GATA-2 (E9T6F) Rabbit mAb 79802 20 µl 51 kDa Rabbit IgG
GATA-3 (D13C9) XP® Rabbit mAb 5852 20 µl 48 kDa Rabbit IgG
GATA-4 (D3A3M) Rabbit mAb 36966 20 µl 55 kDa Rabbit IgG
GATA-6 (D61E4) XP® Rabbit mAb 5851 20 µl 55 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The GATA Transcription Factor Antibody Sampler Kit provides an economical means of evaluating total levels of GATA family proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). Although they are commonly divided as hematopoietic (GATA-1-3) or cardiac (GATA-4-6) factors, GATA proteins are expressed in a wide variety of tissue and play critical roles in embryonic development and organ differentiation (4). GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (5,6). Mutations in the corresponding GATA-1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (7-10). GATA-2 is widely expressed and plays an essential role in many developmental processes (11). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (12). GATA-2 also inhibits the differentiation of white and brown adipocytes and has been shown to suppress the proliferation of neuronal progenitor cells (13-15). GATA-3 is a critical regulator of development and is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (16-19). GATA-3 knockout mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (20). The function of GATA-3 has also been extensively studied in T cell development and has been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (21,22). GATA-4 is crucial for cardiomyocyte differentiation, and not surprisingly, mutations in the GATA-4 gene are implicated in many cardiac diseases, such as tetralogy of Fallot, familial and sporadic dilated cardiomyopathy, and atrial septal defect (23-27). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (28). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (29). GATA-6 plays a critical role in endoderm development and is essential for the development of the heart, gut, and other organs (30-32). Knockout of GATA-6 is embryonic lethal due to defects in the formation of the heart tube and a failure to develop extraembryonic endoderm (30).
 

  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Tremblay, M. et al. (2018) Development 145, dev164384. doi: 10.1242/dev.164384.
  5. Pevny, L. et al. (1991) Nature 349, 257-60.
  6. Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
  7. Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
  8. Cantor, A.B. (2005) Int J Hematol 81, 378-84.
  9. Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
  10. Tubman, V.N. et al. (2007) Blood 109, 3297-9.
  11. Tong, Q. et al. (2003) Drug News Perspect 16, 585-8.
  12. Tsai, F.Y. et al. (1994) Nature 371, 221-6.
  13. Tong, Q. et al. (2005) Mol Cell Biol 25, 706-15.
  14. Tsai, J. et al. (2005) EMBO Rep 6, 879-84.
  15. El Wakil, A. et al. (2006) Development 133, 2155-65.
  16. Debacker, C. et al. (1999) Mech Dev 85, 183-7.
  17. Grote, D. et al. (2008) PLoS Genet 4, e1000316.
  18. Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
  19. Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
  20. Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
  21. Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
  22. Amsen, D. et al. (2007) Immunity 27, 89-99.
  23. Gan, L. et al. (2014) Gene Expr Patterns 16, 8-22.
  24. Yang, Y.Q. et al. (2013) Hum Mutat 34, 1662-71.
  25. Li, R.G. et al. (2013) Biochem Biophys Res Commun 439, 591-6.
  26. Li, J. et al. (2014) Gene 548, 174-81.
  27. Mohan, R.A. et al. (2014) Am J Med Genet A 164A, 2732-8.
  28. Walker, E.M. et al. (2014) Dev Biol 392, 283-94.
  29. LaVoie, H.A. (2014) Biol Reprod 91, 38.
  30. Cai, K.Q. et al. (2008) Dev Dyn 237, 2820-9.
  31. Charron, F. and Nemer, M. (1999) Semin Cell Dev Biol 10, 85-91.
  32. Haveri, H. et al. (2008) BMC Gastroenterol 8, 9.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。
    Revision 1
    #24642

    GATA Transcription Factor Antibody Sampler Kit

    GATA Transcription Factor Antibody Sampler Kit: Image 1 Expand Image
    使用 GATA-3 (D13C9) XP® Rabbit mAb（实线）或浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（虚线）对 THP-1 细胞（蓝色，阴性）和 MCF7 细胞（绿色，阳性）进行流式细胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。
    GATA Transcription Factor Antibody Sampler Kit: Image 2 Expand Image
    使用 GATA-1 (D52H6) XP® Rabbit mAb 对不同细胞系裂解物进行蛋白质印迹分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 3 Expand Image
    使用 GATA-4 (D3A3M) Rabbit mAb（上）和 GAPDH (D16H11) XP® Rabbit mAb #5174（下），对不同细胞系提取物进行蛋白质印迹分析。正如预期的一样，Hela 细胞的 GATA-4 表达非常低。
    GATA Transcription Factor Antibody Sampler Kit: Image 4 Expand Image
    使用 GATA-6 (D61E4) XP® Rabbit mAb 对 Huh7 细胞和 293 细胞提取物进行蛋白质印迹分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 5 Expand Image
    CUT&Tag was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across HNMT, a known target gene of GATA-6 (see our CUT&RUN-qPCR figure).
    GATA Transcription Factor Antibody Sampler Kit: Image 6 Expand Image
    Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 7 Expand Image
    使用 GATA-3 (D13C9) XP® Rabbit mAb 对 CEM 和 SH-SY5Y 细胞提取物进行蛋白质印迹分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 8 Expand Image
    使用 CUT&RUN Assay Kit #86652 对 NK-92 细胞和 GATA-3 (D13C9) XP® Rabbit mAb 或 GATA-3 (E2N1Y) Mouse mAb #96098 进行 CUT&RUN 检测。使用 SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 制备 DNA 文库。该图显示了 GATA-3 的已知靶基因 SH3RF3 内结合（参见包含 CUT&RUN-qPCR 数据的其他结果图）。
    GATA Transcription Factor Antibody Sampler Kit: Image 9 Expand Image
    一抗与靶标蛋白结合之后，与偶联 HRP 的二抗形成复合体。添加 LumiGLO®，在酶催化分解期间发光。
    GATA Transcription Factor Antibody Sampler Kit: Image 10 Expand Image
    对 SH-SY5Y 细胞提取物的 GATA-2 蛋白进行免疫沉淀。泳道 1 为 10% input，泳道 2 为 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900，泳道 3 为 GATA-2 (E9T6F) Rabbit mAb。使用 GATA-2 (E9T6F) Rabbit mAb 进行蛋白质印迹分析。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 用作二抗。
    GATA Transcription Factor Antibody Sampler Kit: Image 11 Expand Image
    使用 GATA-2 (E9T6F) Rabbit mAb（上图）或 β-Actin (D6A8) Rabbit mAb #8457（下图）对不同细胞系的提取物进行蛋白质印迹分析。GATA-2 蛋白在 U-2 OS 细胞和 Jurkat 细胞中阴性表达与预测的表达模式和已发表的观察结果一致。
    GATA Transcription Factor Antibody Sampler Kit: Image 12 Expand Image
    使用 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 对 K-562 细胞的交联染色质和 GATA-2 (E9T6F) Rabbit mAb 或 Normal Rabbit IgG #2729 进行染色质免疫沉淀分析。使用人 GATA2 内含子 4 引物、人 RAP1GAP 内含子 2 引物和 SimpleChIP® Human α Satellite Repeat Primers #4486 通过实时 PCR 对富集的 DNA 进行定量分析。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
    GATA Transcription Factor Antibody Sampler Kit: Image 13 Expand Image
    使用 GATA-2 (E9T6F) Rabbit mAb（实线）或浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（虚线）对 Jurkat 细胞（蓝色，阴性）和 CMK 细胞（绿色，阳性）进行流式细胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。
    GATA Transcription Factor Antibody Sampler Kit: Image 14 Expand Image
    使用 GATA-2 (E9T6F) Rabbit mAb（绿色）、DyLight 650 Phalloidin #12956（红色）和 DAPI #4083（蓝色）对 SH-SY5Y 细胞（左图，阳性）和 U-2 OS 细胞（右图，阴性）进行共聚焦免疫荧光分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 15 Expand Image
    使用 CUT&RUN Assay Kit #86652，以 K562 细胞和 GATA-2 (E9T6F) Rabbit mAb 或 GATA-2 (E8B5C) Rabbit mAb #72445 进行 CUT&RUN。使用 DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795 制备 DNA 库。该图显示合作用遍及 GATA-2 的已知靶标基因 EIF4E（参见包含 CUT&RUN-qPCR 数据的其他结果图）。
    GATA Transcription Factor Antibody Sampler Kit: Image 16 Expand Image
    使用 GATA-1 (D52H6) XP® Rabbit mAb 对石蜡包埋的人骨髓进行免疫组织化学分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 17 Expand Image
    使用 GATA-4 (D3A3M) Rabbit mAb（绿色），对 Huh7（左）和 HeLa（右）细胞进行共聚焦免疫荧光分析。肌动蛋白微丝由 DyLight™ 554 Phalloidin #13054（红色）标记。
    GATA Transcription Factor Antibody Sampler Kit: Image 18 Expand Image
    使用 GATA-6 (D61E4) XP® Rabbit mAb（绿色）对 KM12 细胞（左）和 SK-OV-3 细胞（右）进行共聚焦免疫荧光分析。使用 DY-554 phalloidin（红色）标记肌动蛋白纤丝。
    GATA Transcription Factor Antibody Sampler Kit: Image 19 Expand Image
    CUT&Tag was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across chromosome 2 (upper), including HNMT (lower), a known target gene of GATA-6 (see our CUT&RUN-qPCR figure).
    GATA Transcription Factor Antibody Sampler Kit: Image 20 Expand Image
    Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 21 Expand Image
    使用 GATA-3 (D13C9) XP® Rabbit mAb（左）或 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（右），对石蜡包埋的人乳腺癌进行免疫组织化学分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 22 Expand Image
    使用 CUT&RUN Assay Kit #86652 对 NK-92 细胞和 GATA-3 (D13C9) XP® Rabbit mAb 或 GATA-3 (E2N1Y) Mouse mAb #96098 进行 CUT&RUN 检测。使用 SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 制备 DNA 文库。该图显示了在染色体 2（上图）内的结合，包括 GATA-3 的已知靶标基因 SH3RF3（下图）（参见包含 ChIP-qPCR 数据的其他结果图）。
    GATA Transcription Factor Antibody Sampler Kit: Image 23 Expand Image
    使用 GATA-2 (E9T6F) Rabbit mAb 对来自 CMK 细胞和 PC-12 细胞的提取物进行蛋白质印迹分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 24 Expand Image
    使用 CUT&RUN Assay Kit #86652，以 K562 细胞和 GATA-2 (E9T6F) Rabbit mAb 或 GATA-2 (E8B5C) Rabbit mAb #72445 进行 CUT&RUN。使用 DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795 制备 DNA 库。该图显示了染色体 4（上图）内的结合作用，包括 GATA-2 的已知靶标基因 EIF4E（下图）（参见包含 CUT&RUN-qPCR 数据的其他结果图）。
    GATA Transcription Factor Antibody Sampler Kit: Image 25 Expand Image
    使用 GATA-1 (D52H6) XP® Rabbit mAb 对用福尔马林固定石蜡包埋的且未脱钙的小鼠骨进行免疫组织化学分析。注意：骨髓细胞染色。
    GATA Transcription Factor Antibody Sampler Kit: Image 26 Expand Image
    使用 GATA-4 (D3A3M) Rabbit mAb 对 HeLa 细胞（蓝色）和 ATL-16T 细胞（绿色）进行流式细胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。
    GATA Transcription Factor Antibody Sampler Kit: Image 27 Expand Image
    Flow cytometric analysis of Jurkat cells (blue, negative) and BT-549 cells (green, positive) using GATA-6 (D61E4) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。
    GATA Transcription Factor Antibody Sampler Kit: Image 28 Expand Image
    Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 29 Expand Image
    使用 GATA-3 (D13C9) XP® Rabbit mAb 对石蜡包埋的 MCF-7（左）或 HUVEC（右）细胞沉淀物进行免疫组织化学分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 30 Expand Image
    使用 CUT&RUN Assay Kit #86652 对 NK-92 细胞和 GATA-3 (D13C9) XP® Rabbit mAb 或 Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362 进行 CUT&RUN 检测。使用 human RBM15 promoter primer、human SH3RF3 intron 1 primer 和 SimpleChIP® Human α Satellite Repeat Primers #4486，通过实时 PCR 对富集的 DNA 进行定量分析。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
    GATA Transcription Factor Antibody Sampler Kit: Image 31 Expand Image
    使用 GATA-2 (E9T6F) Rabbit mAb 对 BA/F3 细胞的提取物进行蛋白质印迹分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 32 Expand Image
    使用 CUT&RUN Assay Kit #86652 ，以 K562 细胞和 GATA-2 (E9T6F) Rabbit mAb 或 Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362 进行 CUT&RUN。使用人 EIF4E 启动子引物、人 COX14 启动子引物和 SimpleChIP® Human α Satellite Repeat Primers #4486，通过实时 PCR 对富集的 DNA 进行定量分析。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
    GATA Transcription Factor Antibody Sampler Kit: Image 33 Expand Image
    使用 GATA-1 (D52H6) XP® Rabbit mAb（绿色）和 S6 Ribosomal Protein (54D2) Mouse mAb #2317（红色），对 K-562（左）HeLa（右）细胞进行共聚焦免疫荧光分析。蓝色伪彩 = DRAQ5® #4084（DNA 荧光染料）。
    GATA Transcription Factor Antibody Sampler Kit: Image 34 Expand Image
    使用 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005，对 Caco-2 细胞中提取的交联染色质，在加入 GATA-6 (D61E4) XP® Rabbit mAb 后进行染色质免疫沉淀。使用 DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 制备 DNA 库。本图显示在 FGFR2（GATA-6 的一个已知靶标基因）内的结合作用（参见包含 ChIP-qPCR 数据的其他图）。如需了解其他 ChIP-seq 情况，请下载产品说明书。
    GATA Transcription Factor Antibody Sampler Kit: Image 35 Expand Image
    Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 36 Expand Image
    使用 GATA-3 (D13C9) XP® Rabbit mAb（绿色），对 MCF7（左）和 HUVE（右）细胞进行共聚焦免疫荧光分析。肌动蛋白纤丝用 DY-554 Phalloidin 进行标记（红色）。
    GATA Transcription Factor Antibody Sampler Kit: Image 37 Expand Image
    使用 GATA-1 (D52H6) XP® Rabbit mAb 对 HeLa 细胞（红色）和 K-562 细胞（蓝色）进行流式细胞分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 38 Expand Image
    使用 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005，对 Caco-2 细胞中提取的交联染色质，在加入 GATA-6 (D61E4) XP® Rabbit mAb 后进行染色质免疫沉淀。使用 DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 制备 DNA 库。该结果图显示在染色体 10（上图）内的结合，包括 GATA-6 的已知靶基因 FGFR2（下图）（参见包含 ChIP-qPCR 数据的其他结果图）。
    GATA Transcription Factor Antibody Sampler Kit: Image 39 Expand Image
    Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 40 Expand Image
    使用 GATA-3 (D13C9) XP® Rabbit mAb 对 THP-1 细胞（蓝色） 和 MCF7 细胞（绿色）进行流式细胞分析。
    GATA Transcription Factor Antibody Sampler Kit: Image 41 Expand Image
    使用 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003，对 Caco-2 细胞中提取的交联染色质，在加入 GATA-6 (D61E4) XP® Rabbit mAb 或 Normal Rabbit IgG #2729 后进行染色质免疫沉淀。使用 SimpleChIP® Human FDPS Promoter Primers #13840、人 FGFR2 启动子引物和 SimpleChIP® Human α Satellite Repeat Primers #4486，通过实时 PCR 对富集的 DNA 进行定量。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
    GATA Transcription Factor Antibody Sampler Kit: Image 42 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 43 Expand Image
    使用 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003，对 NK-92 细胞中提取的交联染色质，在加入 GATA-3 (D13C9) XP® Rabbit mAb 或 Normal Rabbit IgG #2729 后进行染色质免疫沉淀。使用 Human CCL4 Promoter Primers、SimpleChIP® Human IFN-γ Promoter Primers #13051 和 SimpleChIP® Human α Satellite Repeat Primers #4486 并通过实时 PCR 对富集的 DNA 进行定量分析。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
    GATA Transcription Factor Antibody Sampler Kit: Image 44 Expand Image
    使用 CUT&RUN Assay Kit #86652，Caco-2 细胞和 GATA-6 (D61E4) XP® Rabbit mAb 进行 CUT&RUN。使用 DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 制备 DNA 库。结果图显示在 FGFR2 基因内结合。
    GATA Transcription Factor Antibody Sampler Kit: Image 45 Expand Image
    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 46 Expand Image
    使用 CUT&RUN Assay Kit #86652，Caco-2 细胞和 GATA-6 (D61E4) XP® Rabbit mAb 进行 CUT&RUN。使用 DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 制备 DNA 库。结果图显示在染色体 10（上图）内的结合，包括 FGFR2 基因（下图）。
    GATA Transcription Factor Antibody Sampler Kit: Image 47 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human stomach using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 48 Expand Image
    使用 CUT&RUN Assay Kit #86652，通过 Caco-2 细胞和 GATA-6 (D61E4) XP® Rabbit mAb 或 Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362 进行 CUT&RUN。使用 人源 HNMT 上游引物和 SimpleChIP® Human α Satellite Repeat Primers #4486 进行实时 PCR 来对富集的 DNA 进行定量。将每份样品中免疫沉淀的 DNA 的量表现为相对于所输入染色质总量（等于 1）的信号。
    GATA Transcription Factor Antibody Sampler Kit: Image 49 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human small intestine using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 50 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human pancreas using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 51 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 52 Expand Image
    >Immunohistochemical analysis of paraffin-embedded normal human lung using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 53 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human heart using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 54 Expand Image
    Immunohistochemical analysis of paraffin-embedded human appendix using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 55 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human adrenal gland using GATA-6 (D61E4) XP ® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 56 Expand Image
    Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 57 Expand Image
    Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 58 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse testis using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 59 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 60 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse colon using GATA-6 (D61E4) XP® Rabbit mAb
    GATA Transcription Factor Antibody Sampler Kit: Image 61 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse small intestine using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 62 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse brain using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 63 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human colon using GATA-6 (D61E4) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
    GATA Transcription Factor Antibody Sampler Kit: Image 64 Expand Image

    Immunohistochemical analysis of paraffin-embedded HuH-7 cell pellet (left, positive) and SUP-B15 cell pellet (right, negative) using GATA-6 (D61E4) XP® Rabbit mAb.

    图像库
    更多了解我们如何获得图像
    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    仅供研究使用。不得用于诊断流程。