WB, IP, IHC-P, ChIP
H
Endogenous
110
Rabbit IgG
#Q08050
2305
Product Information
Product Usage Information
For optimal ChIP results, use 5uL of antibody and 10ug of chromatin (approximately 4 x 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:300 - 1:1200 |
| Chromatin IP | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val699 of human FoxM1 protein.
Background
Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Research studies show that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13).
- Wang, I.C. et al. (2005) Mol Cell Biol 25, 10875-94.
- Leung, T.W. et al. (2001) FEBS Lett 507, 59-66.
- Wang, X. et al. (2002) Proc Natl Acad Sci U S A 99, 16881-6.
- Ma, R.Y. et al. (2005) J Cell Sci 118, 795-806.
- Laoukili, J. et al. (2008) Mol Cell Biol 28, 3076-87.
- Fu, Z. et al. (2008) Nat Cell Biol 10, 1076-82.
- Tan, Y. et al. (2007) Mol Cell Biol 27, 1007-16.
- Anders, L. et al. (2011) Cancer Cell 20, 620-34.
- Ye, H. et al. (1997) Mol Cell Biol 17, 1626-41.
- Barsotti, A.M. and Prives, C. (2009) Oncogene 28, 4295-305.
- Pandit, B. et al. (2009) Cell Cycle 8, 3425-7.
- Pilarsky, C. et al. (2004) Neoplasia 6, 744-50.
- Gartel, A.L. (2008) Expert Opin Ther Targets 12, 663-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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