WB, IP
H M R Mk
Endogenous
145
Rabbit
#Q13045
2314
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Flightless-I protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The flightless-I (fliI) gene was first identified in Drosophila mutant screens for genes involved in flight behavior. Homozygous mutant alleles at the fliI locus are embryonic lethal, whereas heterozygous mutations yield a "flightless" phenotype resulting from defects in flight muscle fiber development (1). The encoded protein (flightless-I, FLII) is a highly conserved member of the gelsolin superfamily, defined by the presence of C-terminal gelsolin motifs that function as actin-binding domains (2). Genetic knock-out studies in mice and worms confirmed that Flightless-I plays a critical and highly conserved role in embryonic development, likely through its effects on actin remodeling of the cytoskeleton (3,4). Postnatally, Flightless-I is recognized to play an important role in wound repair (5). Flightless-I protein levels are increased in many wound types, and depletion of Flightless-I protein levels has been shown to accelerate wound repair by promoting fibroblast proliferation and epithelial migration (6-8). Studies in animal models suggest that Flightless-I may inhibit the wound repair process by modulating TGF-β signaling dynamics in the wound environment (9).
- Miklos, G.L. and De Couet, H.G. (1990) J Neurogenet 6, 133-51.
- Campbell, H.D. et al. (1993) Proc Natl Acad Sci U S A 90, 11386-90.
- Campbell, H.D. et al. (2002) Mol Cell Biol 22, 3518-26.
- Deng, H. et al. (2007) Genetics 177, 847-60.
- Kopecki, Z. and Cowin, A.J. (2008) Int J Biochem Cell Biol 40, 1415-9.
- Cowin, A.J. et al. (2007) J Pathol 211, 572-81.
- Ruzehaji, N. et al. (2012) Eur J Dermatol 22, 740-50.
- Ruzehaji, N. et al. (2013) Biomed Res Int 2013, 389792.
- Adams, D.H. et al. (2009) Br J Dermatol 161, 326-36.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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