WB, IP
H M R Mk
Endogenous
51
Rabbit IgG
#Q13451
2289
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg222 of human FKBP5 protein.
Background
FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for PPIase (peptidylprolyl isomerase) acitivity and allow binding to FK506 and rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The TPR domain of FKBP5 mediates binding to HSP90 complexes (4), as well as glucocorticoid, androgen, and progesterone receptors, which account for its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). In addition, FKBP5 was identified as a negative regulator of Akt, through promotion of Akt - PHLPP interaction and enhanced dephosphorylation of Akt (8).
- Avilés, A. et al. (1995) Clin Oncol (R Coll Radiol) 7, 97-101.
- Fruman, D.A. et al. (1994) FASEB J 8, 391-400.
- Fix, M.L. et al. (2008) Blood Press Monit 13, 199-203.
- Scheufler, C. et al. (2000) Cell 101, 199-210.
- Schülke, J.P. et al. (2010) PLoS One 5, e11717.
- Bouwmeester, T. et al. (2004) Nat Cell Biol 6, 97-105.
- Avellino, R. et al. (2005) Blood 106, 1400-6.
- Pei, H. et al. (2009) Cancer Cell 16, 259-66.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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