Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Firefly Luciferase (E9W9V) Mouse Chimeric mAb #94817
Filter:
- IF
Product Specifications
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse chimeric IgG2a |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Firefly Luciferase (E9W9V) Rabbit mAb #25039.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Firefly Luciferase (E9W9V) Mouse Chimeric mAb recognizes exogenously expressed proteins containing firefly luciferase (P. pyralis).
Species Reactivity:
All Species Expected
Source / Purification
This recombinant chimeric antibody is engineered from Firefly Luciferase (E9W9V) Rabbit mAb #25039 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, Firefly Luciferase (E9W9V) Rabbit mAb #25039, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase (P. pyralis) protein.
The parent antibody, Firefly Luciferase (E9W9V) Rabbit mAb #25039, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase (P. pyralis) protein.
Background
Luciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly, P. pyralis, that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg2+) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase (P. pyralis) protein or its enzymatic activity (2,3).
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For Research Use Only. Not for Use in Diagnostic Procedures.
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