WB, IP
H
Endogenous
32
Rabbit
#Q08830
2267
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn69 of human FGL1 protein. Antibodies are purified by peptide affinity chromatography.
Background
FGL1 (fibrinogen-like protein 1, also known as hepassocin) is a secreted protein mainly expressed in liver and its expression is further enhanced following acute liver injury (1-3). FGL1 null mice have multiple metabolic abnormalities and are more prone to develop hepatocellular carcinoma under certain experimental conditions, suggesting FGL1 has diverse functions in vivo (4,5). More recently, FGL1 was found to be highly expressed in human cancer cells and function as an inhibitory ligand for LAG3. Blockade of the FGL1-LAG3 interaction stimulates tumor immunity in mouse models. In addition, a high plasma FGL1 level in human cancer patients is associated with a poor prognosis and resistance to anti-PD-1/PD-L1 immunotherapy (6).
- Hara, H. et al. (2001) Biochim Biophys Acta 1520, 45-53.
- Liu, Z. and Ukomadu, C. (2008) Biochem Biophys Res Commun 365, 729-34.
- Han, N.K. et al. (2019) Cells 8, pii: E1042. doi: 10.3390/cells8091042.
- Demchev, V. et al. (2013) PLoS One 8, e58084.
- Nayeb-Hashemi, H. et al. (2015) Biochem Biophys Res Commun 465, 167-73.
- Wang, J. et al. (2019) Cell 176, 334-47.e12.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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