WB, IF-IC
H R
Endogenous
15
Rabbit IgG
#O15540
2173
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:2000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val84 of human FABP7 protein.
Background
Fatty acid binding proteins (FABPs) bind to fatty acids and other lipids to function as cytoplasmic lipid chaperones (1). They participate in the transport of fatty acids and other lipids to various cellular pathways (2). Differential expression of FABPs is found in several types of tumors and their normal-cell counterparts (3). FABP7 is abundantly expressed in fetal brain and may be essential for development (4). Expression is required for the establishment of the radial glial fiber system, a system that is necessary for the development of cortical layers (5). Increased expression of FABP7 is associated with reduced survival in patients with glioblastoma (6), and is also found in glial cells following nerve injury (7). Investigators have found loss of FABP7 may be involved in the development and progression of breast cancer and expression of FABP7 has been shown to induce mammary differentiation and to inhibit growth of breast cancer cells (8,9).
- Storch, J. and Thumser, A.E. (2010) J Biol Chem 285, 32679-83.
- Haunerland, N.H. and Spener, F. (2004) Prog Lipid Res 43, 328-49.
- Khan, S.H. and Sorof, S. (1994) Proc Natl Acad Sci U S A 91, 848-52.
- Shimizu, F. et al. (1997) Biochim Biophys Acta 1354, 24-8.
- Feng, L. and Heintz, N. (1995) Development 121, 1719-30.
- Liang, Y. et al. (2005) Proc Natl Acad Sci U S A 102, 5814-9.
- Miller, S.J. et al. (2003) Mol Cell Biol 23, 2213-24.
- Shi, Y.E. et al. (1997) Cancer Res 57, 3084-91.
- Wang, M. et al. (2000) Cancer Res 60, 6482-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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