Revision 1

#43114Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, IHC-P

REACTIVITY:

H M R

SENSITIVITY:

Endogenous

MW (kDa):

95

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q12929

Entrez-Gene Id:

2059

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Eps8 (D1X1L) Rabbit mAb recognizes endogenous levels of total Eps8 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg250 of human Eps8 protein.

Background

Epidermal growth factor receptor pathway substrate 8 (Eps8) is an adaptor protein and can be phosphorylated by several receptor tyrosine kinases including EGFR and Src (1,2). Eps8 is composed of an N-terminal PTB domain, followed by an SH3 domain and a C-terminal effector domain. Eps8 controls actin-based motility by capping the barbed end of actin and bundling actin subunits through its C-terminal effector domain (3,4). The C-terminal α hexlical structure of Eps8 interacts directly with actin to exert these capping and bundling functions (5). The actin capping activity requires the release of Eps8 autoinhibitory binding through SH3 domain interaction with an adaptor molecule, such as Abi-1 (6). This SH3 domain of Eps8 also binds to RN-tre to regulate the down stream Rab5-mediated endocytosis pathway (6). Eps8 functions by binding several receptor tyrosine kinases, such as EGFR or FGFR, to enhance receptor mediated mitogenic Rac signaling and Rab5 endocytosis (6,7). The effector region of Eps8 is necessary for this process. By association with Abi-1 and forming the Eps8/Abi-1/Sos-1 complex, Eps8 couples initial growth factor stimulation to actin motility and the Rac activation pathway (8,9). Eps8 has been shown to be important in the cellular function of filopodial protrusions, cell migration, microvilli formation, and focal adhesion (10-13). Research studies have demonstrated that through its involvement in actin related cellular functions, Eps8 plays a role in cancer cell growth, survival, motility, and invasiveness (14-18).

  1. Fazioli, F. et al. (1993) EMBO J 12, 3799-808.
  2. Gallo, R. et al. (1997) Oncogene 15, 1929-36.
  3. Disanza, A. et al. (2004) Nat Cell Biol 6, 1180-8.
  4. Disanza, A. et al. (2006) Nat Cell Biol 8, 1337-47.
  5. Hertzog, M. et al. (2010) PLoS Biol 8, e1000387.
  6. Lanzetti, L. et al. (2000) Nature 408, 374-7.
  7. Auciello, G. et al. (2013) J Cell Sci 126, 613-24.
  8. Scita, G. et al. (1999) Nature 401, 290-3.
  9. Innocenti, M. et al. (2003) J Cell Biol 160, 17-23.
  10. Welsch, T. et al. (2007) Cancer Lett 255, 205-18.
  11. Frittoli, E. et al. (2011) Immunity 35, 388-99.
  12. Zwaenepoel, I. et al. (2012) Mol Biol Cell 23, 1080-94.
  13. Maa, M.C. et al. (2007) J Biol Chem 282, 19399-409.
  14. Xu, M. et al. (2009) Endocrinology 150, 2064-71.
  15. Chen, Y.J. et al. (2008) Mol Cancer Ther 7, 1376-85.
  16. Yap, L.F. et al. (2009) Oncogene 28, 2524-34.
  17. Chen, H. et al. (2010) Cancer Res 70, 9979-90.
  18. Funato, Y. et al. (2004) Cancer Res 64, 5237-44.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
仅供研究使用。不得用于诊断流程。